Kleihauer-Betke (KB) Test. The KB test is performed to quantitate the number of fetal cells present in the maternal circulation. Once the size of the feto-maternal. Hemoglobin F quantitation by flow cytometry has been found to be simple, reliable, and more precise than the Kleihauer-Betke test,87 In the CAP. The Kleihauer-Betke test is a somewhat crude laboratory method used to screen maternal blood samples for the presence of fetal red blood cells.

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Improvement of the Kleihauer-Betke test by automated detection of fetal erythrocytes in maternal blood. The test is based on the principle that red cells containing fetal hemoglobin HbF are less susceptible to acid elution than cells containing adult hemoglobin HbA.

Kleihauer–Betke test

The indicator cells will be at the center of the rosette, while the fetal RBCs will be clustered around the edges, like petals on a flower.

Kleihauer Betke Test Diann M. General description A peripheral blood smear is made from the maternal postpartum sample and treated with acid. Nitro blue tetrazolium chloride test Complete blood count Absolute neutrophil count.


In 25 women with a negative KB test, bftke had uterine contractions. The KB test is performed to quantitate the number of fetal cells present in the maternal circulation. Support Center Support Center.

This book is distributed under the terms of the Creative Commons Attribution 4. So, unless all stillbirths are tested, a large proportion of FMH will remain undetected. Retrieved from ” https: Then we would conclude that the total percentage of fetal blood lost is approximately.

Kleihauer-Betke (KB) Test – , Laboratory Continuing Education

Kleihauer-betke testing is important in all cases of maternal trauma. See Potential Diagnosis section for preliminary Rosette testing.

Blood film Leucoerythroblastic Blood viscosity Erythrocyte sedimentation rate. Am J Public Health. Reticulocyte index Haptoglobin Mentzer index.

Kleihauer-betke testing is important in all cases of maternal trauma.

Interfering Factors In the case of maternal persistence of fetal hemoglobin or other maternal hemoglobinopathies that result in elevated HbF, the KB test will be falsely positive and flow cytometry must be used to quantitate the amount of fetal hemorrhage in maternal circulation.

Despite its widespread use, the KB test has significant limitations, including Low sensitivity Poor reproducibility.

Cortey A, Brossard Y. Kleihauer Betke Test – StatPearls.

Review [Prevention of fetomaternal rhesus-D allo-immunization. The process exposes maternal blood smear to an acid solution. Home Products Most Popular Contact. KB testing has obstetrical implications in diagnosis and prognosis of preterm labor, fetal demise, and other conditions. Myeloid blood tests CPT — Review Detection of fetomaternal hemorrhage.


It is important to note, however, that such a diagnosis is still not completely conclusive; fetuses losing large quantities of blood over long periods of time are able to compensate for this slower blood loss; since the KB stain tells us nothing with regard to the level of acuity of FMH. None of the trauma scoring systems predicted PTL.


This page was last edited on 21 Augustat Fetal surgery Fetendo Podalic version External cephalic version Amnioinfusion. This is when the Kleihauer-Betke KB test is essential.

PMC ] [ PubMed: No historical or clinical features reliably identify those in whom it may be the cause of an intrauterine death.

HgF, being resistant to the acid, removes intact, whereas HgA is removed. It takes only 0.